The recommended read length is 28 bp for Read 1, 10 bp for Index 1 (i7) and Index 2 (i5) and at least 90 bp for Read 2 (reverse read).ġ0x Genomics ’ Chromium Single Cell Gene Expression Flex runs available on BSSH demo data page Sequencing depth can be adjusted based on the required performance and application. The recommended sequencingĭepth is a minimum 10,000 read pairs/cell. The Chromium Single Cell Gene Expression Flex assay from 10x Genomics is compatible with all Illumina ® platforms, but we recommend using NextSeq™ 550/1000/2000 and NovaSeq™ 6000/X to be able to sequence multiple samples per run. Sequencing libraries are then prepared, sequenced on Illumina high throughput platforms, and analyzed using Cell Ranger and Loupe Browser software tools from 10x Genomics. During GEM generation, the probe sets are ligated and extended to incorporate unique barcodes. Once ready to proceed, samples are hybridized to probe sets and may be processed individually (singleplex workflow) or pooled with up to 16 samples in a single lane of a Chromium chip (multiplex workflow). Samples are fixed and permeabilized and can be safely stored or transported without compromising data quality. Today, we are excited to announce that we have published sequencing data for this product on BaseSpace™ Sequence Hub, including one set of libraries sequenced on the NextSeq™ 2000 P2 flow cell, and another set of libraries sequenced on a NovaSeq™ 6000 SP v1.5 flow cell.įigure 1 - Chromium Single Cell Gene Expression Flex enables an efficient and streamlined workflow for sample management and processing. This assay is compatible with fresh, PFA-fixed, and FFPE samples. Chromium Single Cell Gene Expression Flex assay from 10x Genomics enables gene expression profiling for thousands to hundreds of thousands of cells or nuclei with a sensitive probe-based method that captures the whole human or mouse transcriptome to detect even low-expressing genes. 10x Genomics is a partner that develops multiple single cell and spatial biology solutions. Single cell RNA-sequencing is a next-generation sequencing (NGS) method that examines the transcriptomes of individual cells, providing a high-resolution view of cellular heterogeneity. Series of posts that shed more light on new run data publicly available on BaseSpace ™ Sequence Hub (BSSH) for applications like single cell.
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